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In this lab, an experiment was performed with the primary purpose of analyzing the results with the aim of better understanding the scientific method’s steps. Chemistry’s fundamental knowledge is always necessary for the development of a meaningful understanding of biology. Both organic and inorganic acids are made of nature (Münch and Greenfield 24).
Materials and Methods
In procedure one, 250 ml of distilled water was poured into a 500 ml beaker. Afterward, a small quantity of baking soda was added to the water after which it was stirred with a string rod. At this point, a small amount of phenolphthalein was added to the solution. The solution’s color was then observed.
In experiment two, the materials used for measuring the pH value included the paper, meter, as well as substances for investigation. In the third procedure, a standard for comparison was established by placing seven labeled test tubes in a rack.
Results
Table 1: Standard Solution’s Color in Purple Cabbage Juice Extract
pH
Substance
Color
2
2
yellow
4
4
Yellow
6
6
Yellow
7
7
Yellow
8
8
Blue-green
10
10
Blue-green
12
12
Blue-green
Table 2: Changes of Color in Purple Cabbage Juice and their respective pH value
Well number
Substance
Color change
pH paper value
1
Twist-up
Brown yellow
6
2
Mr. Clean
Blue-green
10
3
Lemon juice
Yellow-orange
5
4
Milk of magnesium
Blue brown
9
5
Diet Coke
Orange
6
6
coffee
Red-brown
7
7
Milk
Cream
7
8
Lime water
Green
12
9
Ammonia
Brown green
10
10
White vinegar
Yellow-orange
2
11
Unknown substance
Brown green
Discussion
A scale of pH ranges from 0 to 14. The acidic solution is the one with pH that is less than 7. Any pH higher than 7 is basic. Also, a neutral substance is one with a pH value of 7 (Münch and Greenfield 29). After adding a small amount of phenolphthalein to the solution, the color changed to pink. Also, the solution was clear when straw was blown steadily. Carbon dioxide was also observed to mix with the solution to turn it clear.
Conclusion
The lab achieved the goal of analyzing the pH results of different materials. Probably, the hydrogen (pH) scale’s potential is the most commonly used method for measuring the substance’s basic or acidic nature.
Work Cited
Münch, Elisabeth v., and Paul F. Greenfield. “Estimating VFA Concentrations in Pre-fermenters by measuring pH.” Water Research, vol. 32, no. 8, 1998, pp. 24-31. Elsevier BV, doi: 10.1016/s0043-1354(97)00469-7.
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